产品货号：
GS2205
中文名称：
Msp20I内切酶
英文名称：
Msp20I
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：Msp20I内切酶，Msp20I限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA(dcm-)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | TGG↑CCA |
| 来源 | Micrococcus species 20 |
| Assayed on | Lanbda DNA(dcm-) |
| Optimal buffer | W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)+?BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);200 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme 80%of the DNA fragments can be ligated and recut.More complete ligation can be reached by using 10%PEG |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Cleaved of DNA is impaired by overlapping Dcm methylation(CmCWGG):TGGCCAGG. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BalI,MlsI,MluNI,MscI |
相关搜索：Msp20I内切酶，Msp20I限制性内切酶